U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

ERX4231718: Sequel sequencing; Raw reads: LINF_isoseqHQreads
1 PACBIO_SMRT (Sequel) run: 17,995 spots, 34.5M bases, 11.9Mb downloads

Design: High quality reads (HQ) of a set of polyadenylated transcripts belonging to the protist Leishmania infantum (JPCM5 strain) that were determined by PacBio sequencing.
Submitted by: Centro Biologia Molecular Severo Ochoa (CBMSO)
Study: Sequences of a set of polyadenylated transcripts belonging to the protist Leishmania infantum (JPCM5 strain) that were determined by PacBio sequencing.
show Abstracthide Abstract
Total RNA from Leishmania infantum (JPCM5 strain) promastigotes was retrotranscribed using poli-T oligonucleotide into cDNA. After a step of PCR amplification, the resulting DNA was sequenced using the Single Molecule, Real-Time (SMRT) Sequencing methodology (PacBio). High quality reads were used to generate circular consensus sequences (CCS), which were classified as complete or truncated transcripts, depending on the presence at 5'-end of the mini-exon (spliced-leader) sequence or not, respectively. Here, we are reporting the sequences of all the transcript isoforms generated in this study.
Sample: High quality reads generated by Iso-seq method. Leishmania infantum JPCM5.
SAMEA6998788 • ERS4766870 • All experiments • All runs
Library:
Instrument: Sequel
Strategy: OTHER
Source: TRANSCRIPTOMIC
Selection: Oligo-dT
Layout: SINGLE
Runs: 1 run, 17,995 spots, 34.5M bases, 11.9Mb
Run# of Spots# of BasesSizePublished
ERR427709117,99534.5M11.9Mb2023-04-05

ID:
27240297

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...